Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents
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چکیده
منابع مشابه
Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents
INTRODUCTION Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nucleoprotein matrixes and cleared from potential inhibitors. The goal of the present work was to ass...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0094886